Genome engineering using CRISPR

Abstract

Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genes using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating and copying the genetic material of interest using recombinant DNA methods or by artificially synthesizing the DNA. A construct is usually created and used to insert this DNA into the host organism.1 Now, a new revolution has seized the scientific community. Within only a few years, research labs worldwide have adopted a new technology that facilitates making specific changes in the DNA of humans, other animals, and plants. This technology is referred to as “CRISPR,” and it has changed not only the way basic research is conducted, but also the way we can now think about treating diseases.2 Now, researchers use the same CRISPR strategy to take on threats like diseases. CRISPR can turn genes on or off, or make them work in a different way, to protect your health